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pe-cyanine5 cd135 (flt3) monoclonal antibody (a2f10)  (Thermo Fisher)


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    Thermo Fisher pe-cyanine5 cd135 (flt3) monoclonal antibody (a2f10)
    Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int <t>Flt3</t> + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .
    Pe Cyanine5 Cd135 (Flt3) Monoclonal Antibody (A2f10), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd135-pe/cd135+a2f10+antibody/pmc12205599-33-0-7
    Average 90 stars, based on 1 article reviews
    pe-cyanine5 cd135 (flt3) monoclonal antibody (a2f10) - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s"

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s

    Journal: iScience

    doi: 10.1016/j.isci.2025.112800

    Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int Flt3 + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .
    Figure Legend Snippet: Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int Flt3 + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .

    Techniques Used: Sequencing, Binding Assay, Genome Wide, Modification, Generated, Transfection, Retroviral, Irradiation, Injection, Flow Cytometry, Isolation, Expressing, Two Tailed Test

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    Article Snippet: For the identification of long-term hematopoietic stem cells (LT-HSCs) and short-term hematopoietic stem cells (ST-HSCs), cells were stained with lineage-PerCPCy5.5 (#558074, BD), Sca-1-APC (#17-5981-82, eBioscience), c-Kit-BV421 (#105828, BioLegend), CD135-PE (#12-1351-83, eBioscience), CD150-PE-Cyanine7 (#25- 1502-82, eBioscience), and CD48-APC-eFluor780 (#47- 0481-82, Invitrogen).

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    Article Snippet: Cytokine receptors were detected by labeling with the following monoclonal antibodies: CD114-biotin, CD116-FITC, CD123-PE, CD131-biotin (all from Pharmingen), CD117-PE (Coulter), and CD135-PE (Caltag).

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    Article Snippet: For the identification of long-term hematopoietic stem cells (LT-HSCs) and short-term hematopoietic stem cells (ST-HSCs), cells were stained with lineage-PerCP-Cy5.5 (#558074, BD), Sca-1-APC (#17-5981-82, eBioscience), c-Kit-BV421 (#105828, BioLegend), CD135-PE (#12-1351-83, eBioscience), CD150-PE-Cyanine7 (#25-1502-82, eBioscience), and CD48-APC-eFluor780 (#47-0481-82, Invitrogen).

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    Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int <t>Flt3</t> + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .
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    Pe Cd135 (Flt3) Monoclonal Antibody (A2f10), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int Flt3 + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .

    Journal: iScience

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s

    doi: 10.1016/j.isci.2025.112800

    Figure Lengend Snippet: Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int Flt3 + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .

    Article Snippet: PE-Cyanine5 CD135 (Flt3) Monoclonal Antibody (A2F10) , eBioscience , Cat# 15-1351-82; RRID: AB_494219.

    Techniques: Sequencing, Binding Assay, Genome Wide, Modification, Generated, Transfection, Retroviral, Irradiation, Injection, Flow Cytometry, Isolation, Expressing, Two Tailed Test

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: G2 arrest primes hematopoietic stem cells for megakaryopoiesis

    doi: 10.1016/j.celrep.2024.114388

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: PE - CD135 (Flt3) Monoclonal Antibody (A2F10) , Thermo Fisher Scientific , Cat# 12-1351-82; RRID: AB_465859.

    Techniques: Recombinant, Adhesive, Purification, Blocking Assay, Amplification, Random Hexamer, Reverse Transcription, SYBR Green Assay, Sterility, Imaging, Software